核磁気共鳴、NMRを用いた絹構造の解明と絹の再生医療材料への応用
メンバー： 朝倉哲郎、中澤靖元、鈴木悠
分野： 生体分子科学、材料化学、生物科学
所属： 工学研究院
キーワード： 絹、再生医療材料、核磁気共鳴
ウェブサイト：
研究概要
絹繊維は、カイコが作るタンパク質ですが、同じ太さの鋼鉄線に匹敵するほど強いのが特徴です。そこで、その原因や繊維化の機構を核磁気共鳴（NMR）を駆使した徹底的な構造解析によって明らかにしてきました。そして、その知見の蓄積をもとにして、絹を用いて小口径人工血管、創傷被覆材、心臓パッチなど、再生医療材料を開発しています。
主要論文・参考事項
1.Tetsuo Asakura, Hiroshi Suzuki and Yasuo Watanabe, Conformational Characterization of Silk Fibroin in Intact Bombyx mori and Philosamia cynthia ricini Silkworms by 13C NMR Spectroscopy, Macromolecules, 16, 1024-1026, 1983.
2.Tetsuo Asakura, Akio Kuzuhara, Ryoko Tabeta and Hazime Saito, Conformation Characterization of Bombyx mori Silk Fibroin in the Solid State by High-Frequency 13C Cross Polarization-Magic Angle Spinning NMR, X-ray Diffraction, and Infrared Spectroscopy, Macromolecules, 18,1841-1845, 1985
3.J. D. van Beek, L. Beaulieu, H. Schäfer, M. Demura, T. Asakura and B. H. Meier, Solid-state NMR determination of the secondary structure of Samia cyｎthia ricini Silk, Nature, 405, 1077-1079 (2000)
4.T. Asakura, J. Ashida, T. Yamane, T. Kameda, Y. Nakazawa, K. Ohgo and K. Komatsu, A repeated β-turn structure inpoly (Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance, J. Mol. Biol., 306, 291-305 ,2001.
5.Enomoto S, Sumi M, Kajimoto K, Nakazawa Y, Takahashi R, Takabayashi C, Asakura T, Sata M, Long-term Patency of Small-diameter Vascular Graft Made from Fibroin, a Silk-based Biodegradable Material, J Vasc Surg., 51, 155-164, 2010.
お問い合わせ先
東京農工大学・先端産学連携研究推進センター
urac[at]ml.tuat.ac.jp（[at]を@に変換してください）
Determination of Silk Structure and Development of silk based biomedical materials
Research members： Dr. Tetsuo Asakura, Dr. Yasumoto Nakazawa, Dr. Yu Suzuki
Research fields： Biomolecular science, Materials chemistry, Biological Science
Departments： Institute of Engineering
Keywords： 絹,再生医療材料,核磁気共鳴
Web site：
Summary
The structure of silk before and after spinning was determined using NMR. On the basis of the accumelated information on structure, we develop silk based small diameter vascular grafts, wound dressings and cardiac patches.
Reference articles and patents
1.Tetsuo Asakura, Hiroshi Suzuki and Yasuo Watanabe, Conformational Characterization of Silk Fibroin in Intact Bombyx mori and Philosamia cynthia ricini Silkworms by 13C NMR Spectroscopy, Macromolecules, 16, 1024-1026, 1983.
2.Tetsuo Asakura, Akio Kuzuhara, Ryoko Tabeta and Hazime Saito, Conformation Characterization of Bombyx mori Silk Fibroin in the Solid State by High-Frequency 13C Cross Polarization-Magic Angle Spinning NMR, X-ray Diffraction, and Infrared Spectroscopy, Macromolecules, 18,1841-1845, 1985
3.J. D. van Beek, L. Beaulieu, H. Schäfer, M. Demura, T. Asakura and B. H. Meier, Solid-state NMR determination of the secondary structure of Samia cyｎthia ricini Silk, Nature, 405, 1077-1079 (2000)
4.T. Asakura, J. Ashida, T. Yamane, T. Kameda, Y. Nakazawa, K. Ohgo and K. Komatsu, A repeated β-turn structure inpoly (Ala-Gly) as a model for silk I of Bombyx mori silk fibroin studied with two-dimensional spin-diffusion NMR under off magic angle spinning and rotational echo double resonance, J. Mol. Biol., 306, 291-305 ,2001.
5.Enomoto S, Sumi M, Kajimoto K, Nakazawa Y, Takahashi R, Takabayashi C, Asakura T, Sata M, Long-term Patency of Small-diameter Vascular Graft Made from Fibroin, a Silk-based Biodegradable Material, J Vasc Surg., 51, 155-164, 2010.
Contact
University Research Administration Center(URAC),
Tokyo University of Agriculture andTechnology
urac[at]ml.tuat.ac.jp
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